Product Type: | Mouse monoclonal IgG3, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IHC-P, IF-Cell |
Clonality: | Monoclonal |
Clone number: | 8-C3 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 18 kDa |
Isotype: | IgG3 |
Immunogen: | Recombinant protein within Human IL31 aa 1-164 / 164. |
Positive control: | A431, LOVO, MCF-7, human tonsil tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:100 1:50-1:200 |
Uniprot #: | SwissProt: Q6EBC2 Human |
Alternative names: | IL 31 IL-31 IL31 IL31_HUMAN Interleukin 31 Interleukin-31 |
Fig1: Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution. | |
Fig2: ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-31 antibody. Counter stained with hematoxylin. |