IL-31 Mouse Monoclonal Antibody [8-C3]
cat.: EM1701-79
Product Type: Mouse monoclonal IgG3, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: 8-C3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 18 kDa
Isotype: IgG3
Immunogen: Recombinant protein within Human IL31 aa 1-164 / 164.
Positive control: Recombinant protein lysate, A431, LOVO, MCF-7, human tonsil tissue, human spleen tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500
1:100
1:50-1:200
Uniprot #: SwissProt: Q6EBC2 Human
Alternative names: IL 31 IL-31 IL31 IL31_HUMAN Interleukin 31 Interleukin-31
Images
EM1701-79_1.jpg Fig1: Western blot analysis of IL-31 on recombinant protein lysate using anti-IL-31 antibody at 1/500 dilution.
EM1701-79_2.jpg Fig2: ICC staining IL-31 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-79_3.jpg Fig3: ICC staining IL-31 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-79_4.jpg Fig4: ICC staining IL-31 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-79_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-31 antibody. Counter stained with hematoxylin.
EM1701-79_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-31 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.