| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 6F5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human AMACR aa 1-382 / 382. |
| Positive control: | human liver tissue lysate, human kidney tissue lysate, mouse liver tissue lysate, rat liver tissue lysate |
| Subcellular location: | Mitochondrion. Peroxisome. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q9UHK6 Human | O09174 Mouse | P70473 Rat |
| Alternative names: | 2 arylpropionyl CoA epimerase 2 methylacyl CoA racemase 2-methylacyl-CoA racemase Alpha methylacyl CoA racemase Alpha methylacyl Coenzyme A racemase Alpha methylacyl-CoA racemase deficiency, included Alpha-methylacyl-CoA racemase Amacr AMACR deficiency, included AMACR_HUMAN CBAS4 Da1-8 EC 5.1.99.4 Macr1 Methylacyl CoA racemase alpha RACE RM P504S |
|
Fig1:
Western blot analysis of AMACR on different lysates with Mouse anti-AMACR antibody (EM1701-80) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human kidney tissue lysate Lane 3: Mouse liver tissue lysate Lane 4: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 300 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-80, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42.4 kDa Observed band size: 42 kDa |