AMACR Mouse Monoclonal Antibody [6F5]
cat.: EM1701-80
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: 6F5
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 42 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human AMACR aa 1-382 / 382.
Positive control: human liver tissue lysate, human kidney tissue lysate, mouse liver tissue lysate, rat liver tissue lysate
Subcellular location: Mitochondrion. Peroxisome.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q9UHK6 Human | O09174 Mouse | P70473 Rat
Alternative names: 2 arylpropionyl CoA epimerase 2 methylacyl CoA racemase 2-methylacyl-CoA racemase Alpha methylacyl CoA racemase Alpha methylacyl Coenzyme A racemase Alpha methylacyl-CoA racemase deficiency, included Alpha-methylacyl-CoA racemase Amacr AMACR deficiency, included AMACR_HUMAN CBAS4 Da1-8 EC 5.1.99.4 Macr1 Methylacyl CoA racemase alpha RACE RM P504S
Images
EM1701-80_1.jpg Fig1: Western blot analysis of AMACR on different lysates with Mouse anti-AMACR antibody (EM1701-80) at 1/1,000 dilution.

Lane 1: Human liver tissue lysate
Lane 2: Human kidney tissue lysate
Lane 3: Mouse liver tissue lysate
Lane 4: Rat liver tissue lysate

Lysates/proteins at 40 µg/Lane.
Exposure time: 300 seconds; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1701-80, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 42.4 kDa
Observed band size: 42 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.