Bcl-2 Mouse Monoclonal Antibody [9F1]
cat.: EM1701-82
Product Type: Mouse monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: 9F1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human BCL2 35-60 aa.
Positive control: THP-1 cell lysates, human tonsil tissue.
Subcellular location: Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:500-1:1,000
Uniprot #: SwissProt: P10415 Human
Alternative names: Apoptosis regulator Bcl 2 Apoptosis regulator Bcl-2 Apoptosis regulator Bcl2 AW986256 B cell CLL/lymphoma 2 B cell leukemia/lymphoma 2 Bcl-2 Bcl2 BCL2_HUMAN C430015F12Rik D630044D05Rik D830018M01Rik Leukemia/lymphoma, B-cell, 2 Oncogene B-cell leukemia 2 PPP1R50 Protein phosphatase 1, regulatory subunit 50 Bcl 2
Images
EM1701-82_1.jpg Fig1: Western blot analysis of Bcl-2 on THP-1 cell lysates with Mouse anti-Bcl-2 antibody (EM1701-82) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 26 kDa
Observed band size: 26 kDa

Exposure time: 1 minute;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-82) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
EM1701-82_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.