Bcl-2 Mouse Monoclonal Antibody [9F1]
cat.: EM1701-82
| Product Type: | Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 9F1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human BCL2 35-60 aa. |
| Positive control: | HL-60 (Human acute promyelocytic leukemia cell) cell lysate, THP-1 (Human acute monoblastic leukemia cell) cell lysate, human tonsil tissue. |
| Subcellular location: | Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P10415 Human |
| Alternative names: | Apoptosis regulator Bcl 2 Apoptosis regulator Bcl-2 Apoptosis regulator Bcl2 AW986256 B cell CLL/lymphoma 2 B cell leukemia/lymphoma 2 Bcl-2 Bcl2 BCL2_HUMAN C430015F12Rik D630044D05Rik D830018M01Rik Leukemia/lymphoma, B-cell, 2 Oncogene B-cell leukemia 2 PPP1R50 Protein phosphatase 1, regulatory subunit 50 Bcl 2 |
Images
|
Fig1:
Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (EM1701-82) at 1/1,000 dilution. Lane 1: HL-60 (Human acute promyelocytic leukemia cell) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-82, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 26 kDa Observed band size: 26 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BCL2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.