CDCP1 Mouse Monoclonal Antibody [A10F10]
cat.: EM1701-84
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: A10F10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 93 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human CDCP1 aa 487-836 / 836.
Positive control: SW480 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate.
Subcellular location: Cell membrane. Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9H5V8 Human | Q5U462 Mouse
Alternative names: 9030022E12Rik AA409659 CD 318 CD_antigen=CD318 CD318 CD318 antigen CDCP 1 Cdcp1 CDCP1_HUMAN CUB domain containing protein 1 CUB domain-containing protein 1 E030027H19Rik FLJ13772 FLJ22375 FLJ22969 Membrane glycoprotein gp140 MGC31813 OTTHUMP00000164120 OTTHUMP00000209568 RGD1305578 SIMA 135 SIMA135 Subtractive immunization M plus HEp3 associated 135 kDa protein Subtractive immunization M plus HEp3-associated 135 kDa protein Transmembrane and associated with src kinases TRASK
Images
EM1701-84_1.jpg Fig1: Western blot analysis of CDCP1 on different lysates with Mouse anti-CDCP1 antibody (EM1701-84) at 1/1,000 dilution.

Lane 1: SW480 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: NIH/3T3 cell lysate (no heat)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 93 kDa
Observed band size: 130 kDa

Exposure time: Lane 1-2: 24 seconds; Lane 3: 10 seconds;
ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1701-84_2.jpg Fig2: Western blot analysis of CDCP1 on different lysates with Mouse anti-CDCP1 antibody (EM1701-84) at 1/1,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HCT 116 cell lysate treated with deglycosylation

Lysates/proteins at 14 µg/Lane.

Predicted band size: 93 kDa
Observed band size: 93/130 kDa (Glycosylated)

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.