CDCP1 Mouse Monoclonal Antibody [A10F10]
cat.: EM1701-84
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A10F10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CDCP1 aa 487-836 / 836. |
| Positive control: | SW480 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Cell membrane. Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9H5V8 Human | Q5U462 Mouse |
| Alternative names: | 9030022E12Rik AA409659 CD 318 CD_antigen=CD318 CD318 CD318 antigen CDCP 1 Cdcp1 CDCP1_HUMAN CUB domain containing protein 1 CUB domain-containing protein 1 E030027H19Rik FLJ13772 FLJ22375 FLJ22969 Membrane glycoprotein gp140 MGC31813 OTTHUMP00000164120 OTTHUMP00000209568 RGD1305578 SIMA 135 SIMA135 Subtractive immunization M plus HEp3 associated 135 kDa protein Subtractive immunization M plus HEp3-associated 135 kDa protein Transmembrane and associated with src kinases TRASK |
Images
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Fig1:
Western blot analysis of CDCP1 on different lysates with Mouse anti-CDCP1 antibody (EM1701-84) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 130 kDa Exposure time: Lane 1-2: 24 seconds; Lane 3: 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CDCP1 on different lysates with Mouse anti-CDCP1 antibody (EM1701-84) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HCT 116 treated with Deglycosylation cell lysate Lysates/proteins at 14 µg/Lane. Predicted band size: 93 kDa Observed band size: 93/130 kDa (Glycosylated) Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.