Zscan4 Mouse Monoclonal Antibody [2-7]
cat.: EM1701-85
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: 2-7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 49 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human ZSCAN4 aa 1-138 / 433.
Positive control: 293 cell lysate transfected with Zscan4, 293 cell lysate, HepG2.
Subcellular location: Nucleus. Telomere.
Recommended Dilutions:
  WB

1:500
Uniprot #: SwissProt: Q8NAM6 Human
Alternative names: FLJ35105 MGC126787 MGC126789 Zinc finger and SCAN domain containing 4 Zinc finger and SCAN domain containing protein 4 Zinc finger and SCAN domain-containing protein 4 Zinc finger protein 494 ZNF 494 ZNF494 ZSCA4_HUMAN ZSCAN4
Images
EM1701-85_1.jpg Fig1: Western blot analysis of Zscan4 on different lysates with Mouse anti-Zscan4 antibody (EM1701-85) at 1/500 dilution.

Lane 1: 293 cell lysate transfected with Zscan4
Lane 2: 293 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 49 kDa
Observed band size: 49 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-85) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.