Zscan4 Mouse Monoclonal Antibody [2-7]
cat.: EM1701-85
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | 2-7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human ZSCAN4 aa 1-138 / 433. |
| Positive control: | 293 cell lysate transfected with Zscan4, 293 cell lysate, HepG2. |
| Subcellular location: | Nucleus. Telomere. |
| Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q8NAM6 Human |
| Alternative names: | FLJ35105 MGC126787 MGC126789 Zinc finger and SCAN domain containing 4 Zinc finger and SCAN domain containing protein 4 Zinc finger and SCAN domain-containing protein 4 Zinc finger protein 494 ZNF 494 ZNF494 ZSCA4_HUMAN ZSCAN4 |
Images
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Fig1:
Western blot analysis of Zscan4 on different lysates with Mouse anti-Zscan4 antibody (EM1701-85) at 1/500 dilution. Lane 1: 293 cell lysate transfected with Zscan4 Lane 2: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-85) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of HepG2 cells with Zscan4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.