PGP9.5 Mouse Monoclonal Antibody [B1-5-6]
cat.: EM1701-86
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: B1-5-6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 25 kDa
Isotype: IgG2a
Immunogen: Synthetic peptide within Human PGP95 aa 174-223 / 223.
Positive control: A-172 cell lysate, SHG-44 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, NCI-H1299 cell lysate, A549 cell lysate, 293T cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY-5Y, rat cerebellum tissue, human pancreas tissue, human kidney tissue, mouse brain tissue, A549.
Subcellular location: Cytoplasm. Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:2,000
1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P09936 Human | Q9R0P9 Mouse | Q00981 Rat
Alternative names: Epididymis luminal protein 117 Epididymis secretory protein Li 53 HEL 117 HEL S 53 NDGOA Neuron cytoplasmic protein 9.5 OTTHUMP00000218137 OTTHUMP00000218139 OTTHUMP00000218140 OTTHUMP00000218141 Park 5 PARK5 PGP 9.5 PGP9.5 PGP95 Protein gene product 9.5 Ubiquitin C terminal esterase L1 Ubiquitin C terminal hydrolase Ubiquitin C terminal hydrolase L1 Ubiquitin carboxyl terminal esterase L1 Ubiquitin carboxyl terminal hydrolase isozyme L1 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Ubiquitin thiolesterase Ubiquitin thiolesterase L1 UCH-L1 UCHL1 UCHL1_HUMAN
Images
EM1701-86_1.jpg Fig1: Western blot analysis of PGP9.5 on different lysates with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/2,000 dilution.

Lane 1: A-172 cell lysate
Lane 2: SHG-44 cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: NCI-H1299 cell lysate
Lane 6: A549 cell lysate
Lane 7: 293T cell lysate
Lane 8: LNCaP cell lysate (negative)
Lane 9: K-562 cell lysate (negative)
Lane 10: Neuro-2a cell lysate
Lane 11: C6 cell lysate
Lane 12: PC-12 cell lysate
Lane 13: Mouse brain tissue lysate
Lane 14: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 25 kDa
Observed band size: 25 kDa

Exposure time: 1 minute 50 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-86) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1701-86_2.jpg Fig2: ICC staining PGP9.5 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-86_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
EM1701-86_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
EM1701-86_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
EM1701-86_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
EM1701-86_7.jpg Fig7: Flow cytometric analysis of A549 cells with PGP9.5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.