Product Type: | Mouse monoclonal IgG2a, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IF-Cell, FC |
Clonality: | Monoclonal |
Clone number: | B1-5-6 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 25 kDa |
Isotype: | IgG2a |
Immunogen: | Synthetic peptide within Human PGP95 aa 174-223 / 223. |
Positive control: | A-172 cell lysate, SHG-44 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, NCI-H1299 cell lysate, A549 cell lysate, 293T cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY-5Y, rat cerebellum tissue, human pancreas tissue, human kidney tissue, mouse brain tissue, A549. |
Subcellular location: | Cytoplasm. Endoplasmic reticulum. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P09936 Human | Q9R0P9 Mouse | Q00981 Rat |
Alternative names: | Epididymis luminal protein 117 Epididymis secretory protein Li 53 HEL 117 HEL S 53 NDGOA Neuron cytoplasmic protein 9.5 OTTHUMP00000218137 OTTHUMP00000218139 OTTHUMP00000218140 OTTHUMP00000218141 Park 5 PARK5 PGP 9.5 PGP9.5 PGP95 Protein gene product 9.5 Ubiquitin C terminal esterase L1 Ubiquitin C terminal hydrolase Ubiquitin C terminal hydrolase L1 Ubiquitin carboxyl terminal esterase L1 Ubiquitin carboxyl terminal hydrolase isozyme L1 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Ubiquitin thiolesterase Ubiquitin thiolesterase L1 UCH-L1 UCHL1 UCHL1_HUMAN |
Fig1:
Western blot analysis of PGP9.5 on different lysates with Mouse anti-PGP9.5 antibody (EM1701-86) at 1/2,000 dilution. Lane 1: A-172 cell lysate Lane 2: SHG-44 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: NCI-H1299 cell lysate Lane 6: A549 cell lysate Lane 7: 293T cell lysate Lane 8: LNCaP cell lysate (negative) Lane 9: K-562 cell lysate (negative) Lane 10: Neuro-2a cell lysate Lane 11: C6 cell lysate Lane 12: PC-12 cell lysate Lane 13: Mouse brain tissue lysate Lane 14: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-86) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining PGP9.5 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin. | |
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin. |
Fig7: Flow cytometric analysis of A549 cells with PGP9.5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |