ASS1 Mouse Monoclonal Antibody [11F1]
cat.: EM1701-87
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 11F1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human ASS1 aa 1-412 / 412. |
| Positive control: | MCF7 cell lysate, Human liver tissue lysate, Human kidney tissue lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate, human colon cancer tissue, mouse testis tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P00966 Human | P16460 Mouse |
| Alternative names: | Argininosuccinate synthase 1 Argininosuccinate synthase Argininosuccinate synthetase 1 ASS Ass-1 ass1 ASSA ASSY_HUMAN Citrulline aspartate ligase Citrulline--aspartate ligase CTLN1 |
Images
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Fig1:
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (EM1701-87) at 1/5,000 dilution. Lane 1: MCF7 cell lysate, 15 µg/Lane Lane 2: Human liver tissue lysate, 30 µg/Lane Lane 3: Human kidney tissue lysate, 30 µg/Lane Lane 4: Mouse liver tissue lysate, 30 µg/Lane Lane 5: Mouse kidney tissue lysate, 30 µg/Lane Lane 6: Rat liver tissue lysate, 30 µg/Lane Lane 7: Rat kidney tissue lysate, 30 µg/Lane Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-87, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46.5 kDa Observed band size: 47 kDa |
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Fig2:
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (EM1701-87) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ASS1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-87) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ASS1 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining ASS1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASS1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.