PLGF Mouse Monoclonal Antibody [3G2]
cat.: EM1701-90
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 3G2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human PLGF aa 1-221. |
| Positive control: | JAR (Human placental choriocarcinoma cell), HepG2, LOVO, rat kidney tissue, mouse testis tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: P49763 Human | P49764 Mouse | Q63434 Rat |
| Alternative names: | D12S1900 Pgf PGFL PIGF Placenta growth factor Placental growth factor Placental growth factor, vascular endothelial growth factor related protein PlGF 2 PlGF PLGF_HUMAN PlGF2 SHGC 10760 |
Images
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Fig1:
Western blot analysis of PLGF on different lysates with Rabbit anti-PLGF antibody (EM1701-90) at 1/500 dilution. Lane 1: JAR (Human placental choriocarcinoma cell) Lysates/proteins at 10 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-90, 1/500 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25 kDa Observed band size: 25 kDa |
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Fig2: ICC staining PLGF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PLGF (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PLGF antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.