ATF6 Mouse Monoclonal Antibody [8D3]
cat.: EM1701-94
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IF-Cell
Clonality: Monoclonal
Clone number: 8D3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 75 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human ATF6 aa 1-670 / 670.
Positive control: HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, HeLa, mouse kidney tissue lysate, rat brain tissue, human placenta tissue, mouse testis tissue, HepG2.
Subcellular location: Endoplasmic reticulum. Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell
1:500-1:5,000
1:50-1:100
1:50-1:100
1:100
Uniprot #: SwissProt: P18850 Human | F6VAN0 Mouse | G3V909 Rat
Alternative names: Activating transcription factor 6 alpha Activating transcription factor 6 ATF 6 ATF6 alpha ATF6 ATF6-alpha ATF6A ATF6A_HUMAN cAMP dependent transcription factor ATF 6 alpha cAMP-dependent transcription factor ATF-6 alpha Cyclic AMP dependent transcription factor ATF 6 alpha DKFZp686P2194 ESTM49 FLJ21663 Processed cyclic AMP dependent transcription factor ATF 6 alpha Processed cyclic AMP-dependent transcription factor ATF-6 alpha
Images
EM1701-94_1.jpg Fig1: Western blot analysis of ATF6 on different lysates with Mouse anti-ATF6 antibody (EM1701-94) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Mouse kidney tissue lysate (40 µg/Lane)

Predicted band size: 75 kDa
Observed band size: 75 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1701-94_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling ATF6 with Mouse anti-ATF6 antibody (EM1701-94) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ATF6 antibody (EM1701-94) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
EM1701-94_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
EM1701-94_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
EM1701-94_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
EM1701-94_6.jpg Fig6: Flow cytometric analysis of HepG2 cells with ATF6 antibody at 1/50 dilution (pink purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.