ERp72 Mouse Monoclonal Antibody [10-F2]
cat.: EM1701-95
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 10-F2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human ERp72 aa 596-645 / 645. |
| Positive control: | A431 cell lysates, HeLa, NIH/3T3, rat testis tissue, human lung cancer tissue, human liver tissue, human placenta tissue, mouse brain tissue, SiHa. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P13667 Human | P08003 Mouse | P38659 Rat |
| Alternative names: | Calcium binding protein intestinal related Endoplasmic reticulum resident protein 70 Endoplasmic reticulum resident protein 72 EPR70 EPR72 ER protein 70 ER protein 72 ERP 70 ERp 72 ERp-72 ERp70 ERp72 PDIA 4 Pdia4 PDIA4_HUMAN PDIR Protein disulfide isomerase A4 Protein disulfide isomerase associated 4 Protein disulfide isomerase family A member 4 protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) Protein disulfide isomerase related protein Protein disulfide-isomerase A4 Protein ERp 72 Protein ERp72 |
Images
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Fig1:
Immunocytochemistry analysis of HeLa cells labeling ERp72 with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling ERp72 with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig8:
Western blot analysis of ERp72 on different lysates with Mouse anti-ERp72 antibody (EM1701-95) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-95, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 73 kDa Observed band size: 73 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.