ERp72 Mouse Monoclonal Antibody [10-F2]
cat.: EM1701-95
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 10-F2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human ERp72 aa 596-645 / 645. |
| Positive control: | A431 cell lysates, HepG2, SKOV-3, rat testis tissue, human lung cancer tissue, human liver tissue, human placenta tissue, mouse brain tissue, SiHa. |
| Subcellular location: | Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P13667 Human | P08003 Mouse | P38659 Rat |
| Alternative names: | Calcium binding protein intestinal related Endoplasmic reticulum resident protein 70 Endoplasmic reticulum resident protein 72 EPR70 EPR72 ER protein 70 ER protein 72 ERP 70 ERp 72 ERp-72 ERp70 ERp72 PDIA 4 Pdia4 PDIA4_HUMAN PDIR Protein disulfide isomerase A4 Protein disulfide isomerase associated 4 Protein disulfide isomerase family A member 4 protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) Protein disulfide isomerase related protein Protein disulfide-isomerase A4 Protein ERp 72 Protein ERp72 |
Images
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Fig1:
Western blot analysis of ERp72 on A431 cell lysates with Mouse anti-ERp72 antibody (EM1701-95) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 73 kDa Observed band size: 73 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-95) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining ERp72 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ERp72 (green) in SKOV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of SiHa cells with ERp72 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.