ERp72 Mouse Monoclonal Antibody [10-F2]
cat.: EM1701-95
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC, FC
Clonality: Monoclonal
Clone number: 10-F2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 73 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human ERp72 aa 596-645 / 645.
Positive control: A431, HepG2, SiHa, SKOV-3, human lung cancer tissue, human liver tissue, rat testis tissue, human placenta tissue, mouse brain tissue.
Subcellular location: Endoplasmic reticulum.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P13667 Human | P08003 Mouse | P38659 Rat
Alternative names: Calcium binding protein intestinal related Endoplasmic reticulum resident protein 70 Endoplasmic reticulum resident protein 72 EPR70 EPR72 ER protein 70 ER protein 72 ERP 70 ERp 72 ERp-72 ERp70 ERp72 PDIA 4 Pdia4 PDIA4_HUMAN PDIR Protein disulfide isomerase A4 Protein disulfide isomerase associated 4 Protein disulfide isomerase family A member 4 protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) Protein disulfide isomerase related protein Protein disulfide-isomerase A4 Protein ERp 72 Protein ERp72
Images
EM1701-95_1.jpg Fig1: Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.
EM1701-95_2.jpg Fig2: ICC staining ERp72 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-95_3.jpg Fig3: ICC staining ERp72 (green) in SKOV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-95_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_9.jpg Fig9: Flow cytometric analysis of SiHa cells with ERp72 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.