ERp72 Mouse Monoclonal Antibody [10-F2]
cat.: EM1701-95
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: 10-F2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human ERp72 aa 596-645 / 645.
Positive control: A431 cell lysates, HeLa, NIH/3T3, rat testis tissue, human lung cancer tissue, human liver tissue, human placenta tissue, mouse brain tissue, SiHa.
Subcellular location: Endoplasmic reticulum.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P13667 Human | P08003 Mouse | P38659 Rat
Alternative names: Calcium binding protein intestinal related Endoplasmic reticulum resident protein 70 Endoplasmic reticulum resident protein 72 EPR70 EPR72 ER protein 70 ER protein 72 ERP 70 ERp 72 ERp-72 ERp70 ERp72 PDIA 4 Pdia4 PDIA4_HUMAN PDIR Protein disulfide isomerase A4 Protein disulfide isomerase associated 4 Protein disulfide isomerase family A member 4 protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) Protein disulfide isomerase related protein Protein disulfide-isomerase A4 Protein ERp 72 Protein ERp72
Images
EM1701-95_1.jpg Fig1: Western blot analysis of ERp72 on A431 cell lysates with Mouse anti-ERp72 antibody (EM1701-95) at 1/2,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 1 minute;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-95) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
EM1701-95_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling ERp72 with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
EM1701-95_3.jpg Fig3: Immunocytochemistry analysis of NIH/3T3 cells labeling ERp72 with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ERp72 antibody (EM1701-95) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
EM1701-95_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin.
EM1701-95_9.jpg Fig9: Flow cytometric analysis of SiHa cells with ERp72 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.