Osteoprotegerin Mouse Monoclonal Antibody [9C1]
cat.: EM1701-98
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 9C1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Osteoprotegerin aa 1-401 / 401. |
| Positive control: | MG-63 cell lysate, Human liver tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, mouse kidney tissue, 293. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:2,000 1:1,000 1:50 |
| Uniprot #: | SwissProt: O00300 Human | O08712 Mouse | O08727 Rat |
| Alternative names: | MGC29565 OCIF OPG Osteoclastogenesis inhibitory factor Osteoprotegerin PDB5 TNF receptor superfamily member 11b TNFRSF 11B TNFRSF11B TR 1 TR1 TR11B_HUMAN Tumor necrosis factor receptor superfamily member 11B |
Images
|
Fig1:
Western blot analysis of Osteoprotegerin on different lysates with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/2,000 dilution. Lane 1: MG-63 cell lysate Lane 2: Human liver tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-98) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunocytochemistry analysis of 293 cells labeling Osteoprotegerin with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.