Osteoprotegerin Mouse Monoclonal Antibody [9C2]
cat.: EM1701-99
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: 9C2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human Osteoprotegerin aa 1-401 / 401.
Positive control: K562 cell lysate, 293, rat lung tissue, human liver tissue, human small intestine tissue, mouse heart tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O00300 Human | O08712 Mouse | O08727 Rat
Alternative names: MGC29565 OCIF OPG Osteoclastogenesis inhibitory factor Osteoprotegerin PDB5 TNF receptor superfamily member 11b TNFRSF 11B TNFRSF11B TR 1 TR1 TR11B_HUMAN Tumor necrosis factor receptor superfamily member 11B
Images
EM1701-99_1.jpg Fig1: Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/500 dilution.
EM1701-99_2.jpg Fig2: Immunocytochemistry analysis of 293 cells labeling Osteoprotegerin with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
EM1701-99_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
EM1701-99_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
EM1701-99_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
EM1701-99_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.