Osteoprotegerin Mouse Monoclonal Antibody [9C2]
cat.: EM1701-99
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 9C2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Osteoprotegerin aa 1-401 / 401. |
| Positive control: | K562 cell lysates, 293, human liver tissue, mouse kidney tissue, rat heart tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:200 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: O00300 Human | O08712 Mouse | O08727 Rat |
| Alternative names: | MGC29565 OCIF OPG Osteoclastogenesis inhibitory factor Osteoprotegerin PDB5 TNF receptor superfamily member 11b TNFRSF 11B TNFRSF11B TR 1 TR1 TR11B_HUMAN Tumor necrosis factor receptor superfamily member 11B |
Images
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Fig1: Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/500 dilution. |
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Fig2:
Immunocytochemistry analysis of 293 cells labeling Osteoprotegerin with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-99) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-99) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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