CD68 Mouse Monoclonal Antibody [F3-D7]
cat.: EM1706-11
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | F3-D7 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 37 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | Human CD68 recombinant protein, CD68-hIgGFc transfected HEK293 cell lysate, U937, Hela, HepG2, human endometrial cancer tissue, human bladder cancer tissue, Jurkat. |
| Subcellular location: | Cell membrane and Endosome membrane. Lysosome membrane. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:2,000 1:200-1:1,000 1:200-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P34810 Human |
| Alternative names: | CD 68 CD68 CD68 antigen CD68 molecule CD68_HUMAN DKFZp686M18236 gp11 Gp110 LAMP4 Macrophage antigen CD68 (microsialin) MACROPHAGE ANTIGEN CD68 Macrosialin SCARD1 Scavenger receptor class D member 1 |
Images
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Fig1: Western blot analysis of CD68 on human CD68 recombinant protein using anti-CD68 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of CD68 on HEK293 (1) and CD68-hIgGFc transfected HEK293 (2) cell lysate using anti-CD68 antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of CD68 on different cell lysate using anti-CD68 antibody at 1/1,000 dilution. Positive control: Line1: U937 Line1: Hela Line2: HepG2 Line3: Jurkat |
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Fig4: ICC staining CD68 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with CD68 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.