| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | H2-E7 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 11.4 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | THP-1 cell lysate, Hela, paraffin-embedded rectum cancer tissues, paraffin-embedded stomach cancer tissues, Raji. |
| Subcellular location: | Nucleus. Chromosome. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:2,000 1:200-1:500 1:200-1:500 1:100-1:200 |
| Uniprot #: | SwissProt: P62805 Human |
| Alternative names: | dJ160A22.1 dJ160A22.2 dJ221C16.1 dJ221C16.9 FO108 H4 H4.k H4/a H4/b H4/c H4/d H4/e H4/g H4/h H4/I H4/j H4/k H4/m H4/n H4/p H4_HUMAN H4F2 H4F2iii H4F2iv H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK H4FM H4FN H4M HIST1H4A HIST1H4B HIST1H4C HIST1H4D HIST1H4E HIST1H4F HIST1H4H HIST1H4I HIST1H4J HIST1H4K HIST1H4L HIST2H4 HIST2H4A Hist4h4 Histone 1 H4a Histone 1 H4b Histone 1 H4c Histone 1 H4d Histone 1 H4e Histone 1 H4f Histone 1 H4h Histone 1 H4i Histone 1 H4j Histone 1 H4k Histone 1 H4l Histone 2 H4a histone 4 H4 Histone H4 MGC24116 |
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Fig1: Western blot analysis of Histone H4 on THP-1 cell lysate using anti- Histone H4 antibody at 1/1,000 dilution. |
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Fig2: ICC staining Histone H4 (green) and Actin filaments (red) in HeLa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using anti- Histone H4 antibody. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti- Histone H4 antibody. |
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Fig5: Flow cytometric analysis of Raji cells with Histone H4 antiboy at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |