BMP4 Mouse Monoclonal Antibody [1C-17]
cat.: EM1706-23
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 1C-17 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 47 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | Human BMP4 recombinant protein, BMP4-hIgGFc transfected HEK293 cell lysate, A549, HepG2, C6, Hela. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IF-cell FC |
1:200-1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P12644 Human |
| Alternative names: | zgc:100779 BMP 2B BMP 4 BMP-2B BMP-4 BMP2B BMP2B1 BMP4 BMP4_HUMAN Bone morphogenetic protein 2B Bone morphogenetic protein 4 DVR4 MCOPS6 MGC100779 OFC11 zbmp-4 ZYME |
Images
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Fig1: Western blot analysis of BMP4 on human BMP4 recombinant protein using anti-BMP4 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of BMP4 on HEK293 (1) and BMP4-hIgGFc transfected HEK293 (2) cell lysate using anti-BMP4 antibody at 1/1,000 dilution. |
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Fig3: Western blot analysis of BMP4 on A549 (1), HepG2 (2), and C6 (3) cell lysate using anti-BMP4 antibody at 1/1,000 dilution. |
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Fig4: ICC staining BMP4 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Flow cytometric analysis of Hela cells with BMP4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.