HSF1 Mouse Monoclonal Antibody [4D-5F]
cat.: EM1706-45
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 4D-5F |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 57 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein |
| Positive control: | Human HSF1 recombinant protein, HSF1-hIgGFc transfected HEK293 cell lysate, A431, human rectum cancer tissue. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IF-cell IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q00613 Human |
| Alternative names: | Heat shock factor 1 Heat shock factor protein 1 Heat shock transcription factor 1 HSF 1 hsf1 HSF1_HUMAN HSTF 1 HSTF1 |
Images
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Fig1: Western blot analysis of HSF1 on human HSF1 recombinant protein using anti-HSF1 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of HSF1 on HEK293 (1) and HSF1-hIgGFc transfected HEK293 (2) cell lysate using anti-HSF1 antibody at 1/1,000 dilution. |
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Fig3: ICC staining HSF1 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-HSF1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.