SNAI2 Mouse Monoclonal Antibody [D5-B6]
cat.: EM1706-65
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | D5-B6 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 30 kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human SNAI2 (AA: 100-200) expressed in E. Coli. |
| Positive control: | Human SNAI2 recombinant protein, SNAI2-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7, human colon cancer tissue, human cervical cancer tissue |
| Subcellular location: | Nucleus,cytoplsm |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:2,000 1:200-1:1,000 1:200-1:1,000 1:200-1:400 |
| Uniprot #: | SwissProt: O43623 Human |
| Alternative names: | MGC10182 Neural crest transcription factor Slug Protein snail homolog 2 Slug ( homolog) zinc finger protein Slug homolog zinc finger protein Slug zinc finger protein SLUGH SLUGH 1 SLUGH1 SNAI 2 Snai2 SNAI2_HUMAN Snail 2 Snail homolog 2 Snail2 WS 2D WS2D Zinc finger protein SLUG Zinc finger protein SNAI2 |
Images
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Fig1: Western blot analysis of SNAI2 on human SNAI2 recombinant protein using anti-SNAI2 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of SNAI2 on HEK293 (1) and SNAI2-hIgGFc transfected HEK293 (2) cell lysate using anti-SNAI2 antibody at 1/1,000 dilution. |
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Fig3: Western blot analysis of SNAI2 on MCF-7 cell lysate using anti-SNAI2 antibody at 1/1,000 dilution. |
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Fig4: ICC staining SNAI2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-SNAI2 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of MCF-7 cells with SNAI2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.