EM1706-67

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human
Applications:	IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	B8-E1

Form:

Liquid

Storage condition:

4℃; -20℃ for long term storage.

Storage buffer:

Purified antibody in PBS with 0.05% sodium azide.

Concentration:

2ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

40 kDa

Isotype:

IgG1

Immunogen:

Synthesized peptide of human MuRF1 (AA: 293-304).

Positive control:

Hela, rectum cancer tissues.muscle tissues.

Subcellular location:

Cytoplasm. Nucleus

Recommended Dilutions:
  IF-cell
  IHC-P
  FC

1:50-1:200
1:50-1:200
1:50-1:100

Uniprot #:

SwissProt: Q969Q1 Human

Alternative names:

E3 ubiquitin-protein ligase TRIM63 FLJ32380 IRF Iris RING finger protein MURF 1 MURF-1 MuRF1 MURF2 Muscle specific ring finger protein 1 Muscle specific ring finger protein 2 Muscle-specific RING finger protein 1 OTTHUMP00000008701 RING finger protein 28 RNF 28 RNF28 SMRZ Striated muscle RING zinc finger protein TRI63_HUMAN TRIM 63 Trim63 Tripartite motif containing 63 tripartite motif containing 63 E3 ubiquitin protein ligase Tripartite motif containing protein 63 Tripartite motif-containing protein 63 Ubiquitin ligase TRIM63

	Fig1: ICC staining MuRF1(green) and Actin filaments(red) inHela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig2: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti-MuRF1 antibody. Counter stained with hematoxylin.
	Fig3: Immunohistochemical analysis of paraffin-embedded huamn muscle tissues using anti-MuRF1 antibody. Counter stained with hematoxylin.
	Fig4: Flow cytometric analysis of Hela cells with MuRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).