WTAP Mouse Monoclonal Antibody [B6-B6]
cat.: EM1706-68
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | B6-B6 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 55 kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli. |
| Positive control: | Human WTAP recombinant protein, WTAP-hIgGFc transfected HEK293 cell lysate, MCF-7, Hela, K562, Hek293, A549, HepG2, Jurkat, Cos7, human cervical cancer tissue, human endometrial cancer tissue. |
| Subcellular location: | Nucleus, nucleolus |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:2,000 1:200-1:1,000 1:200-1:1,000 1:200-1:400 |
| Uniprot #: | SwissProt: Q15007 Human |
| Alternative names: | DKFZp686F20131 Female-lethal(2)D homolog FL2D_HUMAN hFL(2)D KIAA0105 MGC3925 Mum2 OTTHUMP00000017522 OTTHUMP00000017523 PNAS 132 PNAS-132 PNAS132 Pre mRNA splicing regulator WTAP Pre-mRNA-splicing regulator WTAP Putative pre mRNA splicing regulator female lethal 2D homolog Putative pre mRNA splicing regulator female lethal(2D) Putative pre-mRNA splicing regulator female lethal(2D) homolog putative pre-mRNA splicing regulator female-lethal(2D) Wilms tumor 1 associated protein Wilms tumor 1 associating protein Wilms tumor 1-associating protein Wilms' tumor 1 associating protein Wilms' tumour 1-associating protein WT1 associated protein WT1-associated protein WT1-associating protein wtap |
Images
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Fig1: Western blot analysis of WTAP on human WTAP recombinant protein using anti-WTAP antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of WTAP on HEK293 (1) and WTAP-hIgGFc transfected HEK293 (2) cell lysate using anti-WTAP antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of WTAP on different cell lysate using anti-WTAP antibody at 1/1,000 dilution. Positive control: Line1: MCF-7 Line2: Hela Line3: K562 Line4: Hek293 Line5: A549 Line6: HepG2 Line7: Jurkat Line8: Cos7 |
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Fig4: ICC staining WTAP (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-WTAP antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of A549 cells with WTAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.