EM1706-93

Product Type:	Mouse monoclonal IgG2b, primary antibodies
Species reactivity:	Human
Applications:	IF-Cell, FC
Clonality:	Monoclonal
Clone number:	B6-F1

Form:

Liquid

Storage condition:

4℃; -20℃ for long term storage.

Storage buffer:

Purified antibody in PBS with 0.05% sodium azide.

Concentration:

2ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

92 kDa

Isotype:

IgG2b

Immunogen:

Purified recombinant fragment of human PRDM1 (AA: 690-825) expressed in E. Coli.

Positive control:

Human PRDM1 recombinant protein, PRDM1-hIgGFc transfected HEK293 cell lysate, Hela, Raji.

Subcellular location:

Nucleus. Cytoplasm.

Recommended Dilutions:
IF-cell
FC

1:200-1:1,000
1:100-1:200

Uniprot #:

SwissProt: O75626 Human

Alternative names:

B Lymphocyte Induced Maturation Protein 1 Beta interferon gene positive regulatory domain I binding factor Beta-interferon gene positive regulatory domain I-binding factor BLIMP-1 BLIMP1 Positive Regulatory Domain I Binding Factor 1 Positive regulatory domain I-binding factor 1 PR Domain Containing 1 PR domain containing 1 with ZNF domain PR domain containing 1 with ZNF domain isoform 2 PR domain containing protein 1 PR domain zinc finger protein 1 PR domain-containing protein 1 PRDI BF1 PRDI binding factor 1 PRDI-BF1 PRDI-binding factor 1 PRDM 1 Prdm1 PRDM1_HUMAN

	Fig1: Western blot analysis of PRDM1 on human PRDM1 recombinant protein using anti-PRDM1 antibody at 1/1,000 dilution.
	Fig2: Western blot analysis of PRDM1 on HEK293 (1) and PRDM1-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM1 antibody at 1/1,000 dilution.
	Fig3: ICC staining PRDM1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Flow cytometric analysis of Raji cells with PRDM1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).