TRIM25 Mouse Monoclonal Antibody [B12-B5]
cat.: EM1707-34
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | B12-B5 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 71 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein |
| Positive control: | Human TRIM25 recombinant protein, TRIM25-hIgGFc transfected HEK293 cell lysate, MCF-7, K562, A549, MOLT4, Hela, human bladder cancer,human ?rectum cancer. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:200-1:500 1:100-1:200 |
| Uniprot #: | SwissProt: Q14258 Human |
| Alternative names: | E3 ubiquitin/ISG15 ligase TRIM25 EFP Estrogen responsive finger protein Estrogen-responsive finger protein RING finger protein 147 RNF 147 RNF147 TRI25 TRI25_HUMAN TRIM 25 Trim25 Tripartite motif containing 25 Tripartite motif containing protein 25 Tripartite motif protein TRIM25 Tripartite motif-containing protein 25 Tripartite motifcontaining25 Ubiquitin/ISG15-conjugating enzyme TRIM25 Z147 Zinc finger protein 147 (estrogen responsive finger protein) Zinc finger protein 147 Zinc finger protein-147 ZNF 147 ZNF147 |
Images
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Fig1: Western blot analysis of TRIM25 on human TRIM25 recombinant protein using anti-TRIM25 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of TRIM25 on HEK293 (1) and TRIM25-hIgGFc transfected HEK293 (2) cell lysate using anti-TRIM25 antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of TRIM25 on different cell lysate using anti-TRIM25 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: MCF-7 Lane 3: K562 Lane 4: A549 Lane 5: MOLT4 |
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Fig4: ICC staining TRIM25 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-TRIM25 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-TRIM25 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with TRIM25 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.