TLR9 Mouse Monoclonal Antibody [H2-B12]
cat.: EM1707-59
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | H2-B12 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 116 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein |
| Positive control: | Human TLR9 recombinant protein, TLR9-hIgGFc transfected HEK293 cell lysate, SK-OV-3, A549, human ?ovarian cancer tissue, human endometrial cancer tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. Cell membrane |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q9NR96 Human |
| Alternative names: | CD 289 CD289 TLR 9 TLR9 TLR9_HUMAN Toll like receptor 9 Toll like receptor 9 isoform A precursor Toll like receptor 9 isoform B Toll-like receptor 9 |
Images
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Fig1: Western blot analysis of TLR9 on human TLR9 recombinant protein using anti-TLR9 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of TLR9 on HEK293 (1) and TLR9-hIgGFc transfected HEK293 (2) cell lysate using anti-TLR9 antibody at 1/1,000 dilution. |
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Fig3: ICC staining TLR9 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-TLR9 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-TLR9 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of A549 cells with TLR9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.