PD1 Mouse Monoclonal Antibody [1F2]
cat.: EM1707-60
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: 1F2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: 32 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human PD1 aa 1-200 / 288.
Positive control: Human tonsil tissue, Mouse spleen tissue, Jurkat, recombinant PD1 protein.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: Q15116 Human
Alternative names: CD279 CD279 antigen hPD 1 hPD l hPD-1 hSLE1 PD 1 PD-1 PD1 PDCD 1 PDCD1 PDCD1_HUMAN Programmed cell death 1 Programmed cell death 1 protein Programmed cell death protein 1 Protein PD 1 Protein PD-1 SLEB2 Systemic lupus erythematosus susceptibility 2
Images
EM1707-60_1.jpg Fig1: Western blot analysis of PD1 on PD1 transfected 293FT cell lysate using anti-PD1 antibody at 1/1,000 dilution.
EM1707-60_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD1 antibody. Counter stained with hematoxylin.
EM1707-60_3.jpg Fig3: Flow cytometric analysis of Jurkat cells with PD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.