Product Type: | Mouse monoclonal IgG1, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | 1F2 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 32 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within Human PD1 aa 1-200 / 288. |
Positive control: | Human tonsil tissue, Mouse spleen tissue, Jurkat, recombinant PD1 protein. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:100-1:500 1:50-1:100 |
Uniprot #: | SwissProt: Q15116 Human |
Alternative names: | CD279 CD279 antigen hPD 1 hPD l hPD-1 hSLE1 PD 1 PD-1 PD1 PDCD 1 PDCD1 PDCD1_HUMAN Programmed cell death 1 Programmed cell death 1 protein Programmed cell death protein 1 Protein PD 1 Protein PD-1 SLEB2 Systemic lupus erythematosus susceptibility 2 |
Fig1: Western blot analysis of PD1 on PD1 transfected 293FT cell lysate using anti-PD1 antibody at 1/1,000 dilution. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD1 antibody. Counter stained with hematoxylin. | |
Fig3: Flow cytometric analysis of Jurkat cells with PD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |