RAD50 Mouse Monoclonal Antibody
cat.: EM1707-66
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 154 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein |
| Positive control: | Human RAD50 recombinant protein, RAD50-hIgGFc transfected HEK293 cell lysate, C6, HepG2, Hela, human esophageal cancer tissue, human rectum cancer tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: Q92878 Human |
| Alternative names: | DNA repair protein RAD50 hRAD50 hsRAD50 Mrell NBSLD Rad 50 RAD50 2 rad50 RAD50 homolog (S cerevisiae) RAD50 homolog RAD50 PEN RAD50 S. cerevisiae homolog of RAD50_HUMAN RAD502 Rad50l Truncated RAD50 protein |
Images
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Fig1: Western blot analysis of RAD50 on human RAD50 recombinant protein using anti-RAD50 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of RAD50 on HEK293 (1) and RAD50-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution. |
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Fig3: Western blot analysis of RAD50 on C6 (1) and HepG2 (2) cell lysate using anti-RAD50 antibody at 1/1,000 dilution. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-RAD50 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with RAD50 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.