RALA Mouse Monoclonal Antibody
cat.: EM1707-77
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 24 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | Human RALA recombinant protein, RALA-hIgGFc transfected HEK293 cell lysate, HepG2, MCF-7, A549, K562, Raji, MOLT4, Hela, COS7, NIH/3T3, human bladder cancer tissue, human ovarian cancer tissue. |
| Subcellular location: | Cell surface. Cell membrane. Cleavage furrow. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P11233 Human |
| Alternative names: | MGC48949 Ral a Ral A protein RAL RALA RALA_HUMAN Ras family small GTP binding protein RALA RAS like protein A Ras related protein RalA Ras-related protein Ral-A v ral simian leukemia viral oncogene homolog A (ras related) v ral simian leukemia viral oncogene homolog A |
Images
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Fig1: Western blot analysis of RALA on human RALA recombinant protein using anti-RALA antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of RALA on HEK293 (1) and RALA-hIgGFc transfected HEK293 (2) cell lysate using anti-RALA antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of RALA on different cell lysate using anti-RALA antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: MCF-7 Lane 3: A549 Lane 4: K562 Lane 5: Raji Lane 6: MOLT4 Lane 7: Hela Lane 8: COS7 Lane 9: NIH/3T3 |
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Fig4: ICC staining RALA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-RALA antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RALA antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of MCF-7 cells with RALA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.