GLI1 Mouse Monoclonal Antibody [F8-B9]
cat.: EM1708-22
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | F8-B9 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 118 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | Human GLI1 recombinant protein, GLI1-hIgGFc transfected HEK293 cell lysate, Hela, HepG2, human ovarian cancer tissue, human esophageal cancer tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
IF-cell IHC-P FC |
1:50-1:200 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P08151 Human |
| Alternative names: | Gli 1 GLI GLI family zinc finger 1 GLI Kruppel family member 1 gli1 GLI1_HUMAN Glioma associated oncogene 1 Glioma associated oncogene homolog 1 (zinc finger protein) Glioma associated oncogene homolog Glioma-associated oncogene Oncogene GLI Zfp 5 Zfp5 Zinc finger protein GLI 1 Zinc finger protein GLI1 |
Images
|
Fig1: ICC staining GLI1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-GLI1 antibody. Counter stained with hematoxylin. |
|
Fig4: Flow cytometric analysis of HepG2 cells with GLI1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.