ESR1 Mouse Monoclonal Antibody [E8-A6]
cat.: EM1708-51
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | E8-A6 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 66 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | Human ESR1 recombinant protein, ESR1-hIgGFc transfected HEK293 cell lysate, MOLT4, Raji, MCF-7, T47D, SK-Br-3, Hela, C6 cell lysate, Hela, human breast cancer tissue, human esophageal cancer tissue. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P03372 Human | P06211 Rat |
| Alternative names: | 7*/654 isoform 7*/819 2 isoform 7*/822 isoform 8*/901 isoform 8*/941 isoform DKFZp686N23123 ER alpha ER ER-alpha Era ESR ESR1 ESR1_HUMAN ESRA Estradiol receptor Estrogen nuclear receptor alpha Estrogen receptor 1 Estrogen receptor alpha 3* 4 5 6 7*/822 isoform Estrogen receptor alpha Estrogen receptor alpha delta 3* 4 5 6 7* 8*/941 isoform Estrogen receptor alpha delta 3* 4 5 6 7*/819 2 isoform Estrogen receptor alpha delta 4 +49 isoform Estrogen receptor alpha delta 4* 5 6 7*/654 isoform Estrogen receptor alpha delta 4* 5 6 7 8*/901 isoform Estrogen receptor alpha E1 E2 1 2 Estrogen receptor alpha E1 N2 E2 1 2 Estrogen receptor ESTRR NR3A1 Nuclear receptor subfamily 3 group A member 1 |
Images
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Fig1: Western blot analysis of ESR1 on human ESR1 recombinant protein using anti-ESR1 antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of ESR1 on HEK293 (1) and ESR1-hIgGFc transfected HEK293 (2) cell lysate using anti-ESR1 antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of ESR1 on different cell lysate using anti-ESR1 antibody at 1/1,000 dilution. Positive control: Line1: MOLT4 Line2: Raji Line3: MCF-7 Line4: T47D Line5: SK-Br-3 Line6: Hela Line7: C6 |
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Fig4: ICC staining ESR1 (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-ESR1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-ESR1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with ESR1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.