IL3RA Mouse Monoclonal Antibody [C5-E11]
cat.: EM1709-19
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | C5-E11 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 43 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | Human IL3RA recombinant protein, IL3RA-hIgGFc transfected HEK293 cell lysate, human ovarian cancer tissue, human lung cancer tissue, Hela. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P26951 Human |
| Alternative names: | CD123 CD123 antigen hIL 3Ra hIL-3Ra hIL3Ra IL 3 receptor alpha SP2 isoform IL 3R alpha IL-3 receptor subunit alpha IL-3R subunit alpha IL-3R-alpha IL-3RA IL3 Receptor alpha IL3R Il3ra IL3RA_HUMAN IL3RAX IL3RAY IL3RX IL3RY Interleukin 3 receptor alpha chain Interleukin 3 receptor alpha (low affinity) Interleukin 3 receptor alpha Interleukin-3 Receptor alpha Interleukin-3 receptor subunit alpha Interleukin3 receptor Interleukin3 receptor Y-chromosomal |
Images
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Fig1: Western blot analysis of IL3RA on human IL3RA recombinant protein using anti-IL3RA antibody at 1/1,000 dilution. |
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Fig2: Western blot analysis of IL3RA on HEK293 (1) and IL3RA-hIgGFc transfected HEK293 (2) cell lysate using anti-IL3RA antibody at 1/1,000 dilution. |
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Fig3: ICC staining IL3RA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with IL3RA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.