RICTOR Mouse Monoclonal Antibody [E9-B3]
cat.: EM1709-50
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | E9-B3 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 192 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human RICTOR aa 1502-1708 / 1708. |
| Positive control: | Hela, PANC-1, MOLT4, HepG2, HEK293, NIH-3T3, human thyroid gland tissue, human breast carcinoma tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: Q6R327 Human | Q6QI06 Mouse |
| Alternative names: | AVO3 AVO3 homolog DKFZp686B11164 hAVO3 KIAA1999 Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) mAVO3 MGC39830 PIA Pianissimo Rapamycin insensitive companion of mTOR Rapamycin-insensitive companion of mTOR Rictor RICTR RICTR_HUMAN RPTOR independent companion of MTOR complex 2 TORC2 specific protein AVO3 |
Images
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Fig1:
Western blot analysis of RICTOR on different cell lysates using anti-RICTOR antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PANC-1 Lane 3: MOLT4 Lane 4: HepG2 Lane 5: HEK293 |
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Fig2: ICC staining RICTOR (green) and actin filaments (red) in NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-RICTOR antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RICTOR antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of Hela cells with RICTOR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.