FYN Mouse Monoclonal Antibody [D2-A10]
cat.: EM1709-88
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | D2-A10 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 61 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | Human FYN recombinant protein, U251, breast cancer tissue, brain tissue, Hela. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P06241 Human |
| Alternative names: | C syn protooncogene Fyn FYN oncogene related to SRC FGR YES FYN_HUMAN OKT3 induced calcium influx regulator P59 FYN p59-Fyn Protein tyrosine kinase fyn Proto oncogene tyrosine protein kinase fyn Proto-oncogene c-Fyn Proto-oncogene Syn Protooncogene Syn SLK Src like kinase Src yes related novel gene Src-like kinase Src/yes related novel SYN Tyrosine kinase p59fyn T Tyrosine kinase p59fyn(T) Tyrosine-protein kinase Fyn |
Images
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Fig1: Western blot analysis of FYN on human FYN recombinant protein using anti-FYN antibody at 1/1,000 dilution. |
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Fig2: ICC staining FYN (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-FYN antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-FYN antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of Hela cells with FYN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.