CD105 Mouse Monoclonal Antibody [H4-A9]
cat.: EM1709-99
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | H4-A9 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 71 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | CD105-hIgGFc transfected HEK293 cell lysate, HepG2, human kidney cancer tissue, human stomach cancer tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-cell IHC-P FC |
1:500-1:1,000 1:100-1:500 1:100-1:500 1:100-1:200 |
| Uniprot #: | SwissProt: P17813 Human |
| Alternative names: | AI528660 AI662476 CD 105 CD105 CD105 antigen EGLN_HUMAN END Endoglin Eng FLJ41744 HHT1 ORW ORW1 Osler Rendu Weber syndrome 1 RP11 228B15.2 S endoglin S-endoglin SN6 |
Images
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Fig1: Western blot analysis of CD105 on HEK293 (1) and CD105-hIgGFc transfected HEK293 (2) cell lysate using anti-CD105 antibody at 1/1,000 dilution. |
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Fig2: ICC staining CD105 (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue using anti-CD105 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-CD105 antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of HepG2 cells with CD105 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.