CD100 Mouse Monoclonal Antibody [5H6E3]
cat.: EM1710-47
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 5H6E3 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 96.2kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human CD100 (AA: extra 590-734) expressed in E. Coli. |
| Positive control: | K562 cells 、Ramos cells 、cervical cancer tissues 、bladder cancer tissues |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: Q92854 Human |
| Alternative names: | A8 BB 18 BB18 CD 100 CD100 CD100 antigen Coll 4 COLL4 Collapsin 4 Collapsin4 GR3 Leukocyte activation antigen CD100 M sema G MSEMA SEM4D_HUMAN Sema 4d Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D Sema H SEMA J Sema4d Semacl 2 Semacl2 SemaH SEMAJ Semaphorin 4D Semaphorin C like 2 Semaphorin H Semaphorin J Semaphorin-4D Semaphorin4D SemaphorinJ Semcl 2 Semcl2 |
Images
|
Fig1: Western blot analysis of CD100 against human CD100 (AA: extra 590-734) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of CD100 against HEK293 (1) and CD100 (AA: extra 590-734)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-CD100 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-47, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CD100 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1710-47, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Flow cytometric analysis of CD100 was done on Ramos cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
|
Fig6: Flow cytometric analysis of CD100 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-47, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.