BTN2A2 Mouse Monoclonal Antibody [4B11C8]
cat.: EM1710-73
| Product Type: | Mouse monoclonal IGg2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 4B11C8 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 59kDa |
| Isotype: | IGg2a |
| Immunogen: | Purified recombinant fragment of human BTN2A2 (AA: extra 57-237) expressed in E. Coli. |
| Positive control: | K562 cell lysate, Hela cells |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-cell FC |
1:500-1:2,000 1：50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: Q8WVV5 Human |
| Alternative names: | BT2.2 BT2A2_HUMAN BTF2 BTN2A2 butyrophilin 2 Butyrophilin subfamily 2 member A2 |
Images
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Fig1: Western blot analysis of BTN2A2 against human BTN2A2 (AA: extra 57-237) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of BTN2A2 against HEK293 (1) and BTN2A2 (AA: extra 57-237)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3: Western blot analysis of BTN2A2 against K562 (1) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig4: Immunocytochemistry staining of BTN2A2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-73, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue), Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red). |
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Fig5: Flow cytometric analysis of BTN2A2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-73, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.