ATXN1 Mouse Monoclonal Antibody [4C7B11]
cat.: EM1711-76
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 4C7B11 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 86.9kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human ATXN1 (AA: 645-815) expressed in E. Coli. |
| Positive control: | C6, COS7, NIH/3T3, and HL-60 cell lysate, Jurkat cells, endometrial cancer tissues |
| Subcellular location: | Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P54253 Human |
| Alternative names: | alternative ataxin1 Ataxin-1 ATX1 ATX1_HUMAN Atxn1 D6S504E OTTHUMP00000016065 SCA1 Spinocerebellar ataxia type 1 protein |
Images
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Fig1: Western blot analysis of ATXN1 against human ATXN1 (AA: 645-815) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of ATXN1 against HEK293 (1) and ATXN1 (AA: 645-815)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3: Western blot analysis of ATXN1 against C6 (1), COS7 (2), NIH/3T3 (3), and HL-60 (4) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig4: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using anti-ATXN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1711-76, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of ATXN1 was done on Jurkat cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-76, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.