CD307C Mouse Monoclonal Antibody [4B4B2]
cat.: EM1711-77
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | 4B4B2 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 80.9kDa |
| Isotype: | IgG2a |
| Immunogen: | Purified recombinant fragment of human CD307C (AA: extra 18-153) expressed in E. Coli. |
| Positive control: | HL-60 cells |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:100-1:200 |
| Uniprot #: | SwissProt: Q96P31 Human |
| Alternative names: | CD307c Fc receptor homolog 3 Fc receptor-like protein 3 FcR-like protein 3 FcRH3 FcRL3 FCRL3_HUMAN hIFGP3 IFGP family protein 3 IFGP3 Immune receptor translocation-associated protein 3 Immunoglobulin superfamily receptor translocation associated protein 3 IRTA3 SH2 domain-containing phosphatase anchor protein 2 SPAP2 |
Images
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Fig1: Western blot analysis of CD307C against human CD307C (AA: extra 18-153) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of CD307C against HEK293 (1) and CD307C (AA: extra 18-153)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Flow cytometric analysis of CD307C was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-77, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.