CD215 Mouse Monoclonal Antibody [5F10D9]
cat.: EM1712-22
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 5F10D9 |
| Form: | Liquid |
| Storage condition: | 4℃; -20℃ for long term storage. |
| Storage buffer: | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 28.2kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human CD215 (AA: extra 31-205) expressed in E. Coli. |
| Positive control: | LOVO and HCT116 cell lysate |
| Subcellular location: | Secreted. Nucleus membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. |
| Recommended Dilutions:
WB IF-cell |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q13261 Human |
| Alternative names: | AA690181 CD215 I15RA_HUMAN IL 15R alpha IL-15 receptor subunit alpha IL-15R-alpha IL-15RA Il15ra Interleukin 15 receptor alpha Interleukin 15 receptor subunit alpha MGC104179 sIL-15 receptor subunit alpha sIL-15R-alpha sIL-15RA Soluble interleukin 15 receptor subunit alpha Soluble interleukin-15 receptor subunit alpha |
Images
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Fig1: Western blot analysis of CD215 against human CD215 (AA: extra 31-205) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of CD215 against HEK293 (1) and CD215 (AA: extra 31-205)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Western blot analysis of CD215 against LOVO (1) and HCT116 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.