IMP3 Mouse Monoclonal Antibody [4-C2-5]
cat.: EM1801-01
| Product Type: | Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | 4-C2-5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IMP3 aa 350-579. |
| Positive control: | HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, Mouse spleen tissue lysate, Mouse placenta tissue lysate, Rat spleen tissue lysate, Rat placenta tissue lysate, PANC-1. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: O00425 Human | Q9CPN8 Mouse Entrez Gene: 315697 Rat |
| Alternative names: | Cancer/testis antigen 98 CT98 DKFZp686F1078 hKOC IF2B3_HUMAN IGF II mRNA binding protein 3 IGF-II mRNA-binding protein 3 IGF2 mRNA binding protein 3 IGF2 mRNA-binding protein 3 IGF2BP3 IMP 3 IMP-3 Insulin like growth factor 2 mRNA binding protein 3 Insulin-like growth factor 2 mRNA-binding protein 3 KH domain containing protein overexpressed in cancer KH domain-containing protein overexpressed in cancer KOC 1 KOC1 VICKZ 3 VICKZ family member 3 VICKZ3 |
Images
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Fig1:
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Mouse placenta tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Lane 7: Rat placenta tissue lysate (40 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: Lane 1-4: 20 seconds; Lane 5-6: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of PANC-1 cells with IMP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.