EM1801-02

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human, Rat
Applications:	IHC-P, FC
Clonality:	Monoclonal
Clone number:	2A1

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2ug/ul

Purification:

Protein G affinity purified.

Molecular weight:

Predicted band size: 82 kDa

Isotype:

IgG1

Immunogen:

Recombinant protein within Human Calpain 1 aa 1-714 / 714.

Positive control:

Rat testis tissue, human colon cancer tissue, human spleen tissue, A431.

Subcellular location:

Plasma membrane.

Recommended Dilutions:
IHC-P
FC

1:50-1:200
1:50-1:100

Uniprot #:

SwissProt: P07384 Human | P97571 Rat

Alternative names:

Ca2 activated neutral protease Calcium activated neutral proteinase Calcium activated neutral proteinase small subunit Calcium dependent protease small subunit 1 Calcium dependent protease small subunit Calcium-activated neutral proteinase 1 Calpain 1 Calpain 1 large subunit Calpain 1, (mu/I) large subunit Calpain mu type Calpain mu-type Calpain regulatory subunit Calpain small subunit 1 Calpain, large polypeptide L1 Calpain-1 catalytic subunit Calpain-1 large subunit CAN1_HUMAN CANP 1 CANP CANP small subunit CANP1 CANPL 1 CANPL1 CAPN 1 CAPN1 Cell proliferation inducing protein 30 Cell proliferation-inducing gene 30 protein Micromolar Calpain Micromolar-calpain Mu Calpain muCANP muCL

	Fig1: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
	Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
	Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
	Fig4: Flow cytometric analysis of A431 cells with Calpain 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.