Calpain 1 Mouse Monoclonal Antibody [2A2]
cat.: EM1801-03
Product Type: Mouse monoclonal IgM, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: 2A2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein L affinity purified.
Molecular weight: 82 kDa
Isotype: IgM
Immunogen: Recombinant protein.
Positive control: Rat testis tissue, human colon cancer tissue, human kidney tissue, mouse fallopian tubes tissue, A431, SiHa.
Subcellular location: Plama membrane.
Recommended Dilutions:
  ICC
  IHC-P
  FC

1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P07384 Human | O35350 Mouse | P97571 Rat
Alternative names: Ca2 activated neutral protease Calcium activated neutral proteinase Calcium activated neutral proteinase small subunit Calcium dependent protease small subunit 1 Calcium dependent protease small subunit Calcium-activated neutral proteinase 1 Calpain 1 Calpain 1 large subunit Calpain 1, (mu/I) large subunit Calpain mu type Calpain mu-type Calpain regulatory subunit Calpain small subunit 1 Calpain, large polypeptide L1 Calpain-1 catalytic subunit Calpain-1 large subunit CAN1_HUMAN CANP 1 CANP CANP small subunit CANP1 CANPL 1 CANPL1 CAPN 1 CAPN1 Cell proliferation inducing protein 30 Cell proliferation-inducing gene 30 protein Micromolar Calpain Micromolar-calpain Mu Calpain muCANP muCL
Images
EM1801-03_1.jpg Fig1: ICC staining Calpain 1(green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1801-03_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
EM1801-03_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
EM1801-03_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
EM1801-03_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
EM1801-03_6.jpg Fig6: Flow cytometric analysis of A431 cells with Calpain 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.