Calpain 1 Mouse Monoclonal Antibody [2A2]
cat.: EM1801-03
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 2A2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein L affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within Human Calpain 1 aa 1-714 / 714. |
| Positive control: | Rat testis tissue, human spleen tissue, human colon cancer tissue, human kidney tissue, mouse fallopian tubes tissue, A431, SiHa. |
| Subcellular location: | Cell membrane, Cytoplasm, Membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P07384 Human | O35350 Mouse | P97571 Rat |
| Alternative names: | Ca2 activated neutral protease Calcium activated neutral proteinase Calcium activated neutral proteinase small subunit Calcium dependent protease small subunit 1 Calcium dependent protease small subunit Calcium-activated neutral proteinase 1 Calpain 1 Calpain 1 large subunit Calpain 1, (mu/I) large subunit Calpain mu type Calpain mu-type Calpain regulatory subunit Calpain small subunit 1 Calpain, large polypeptide L1 Calpain-1 catalytic subunit Calpain-1 large subunit CAN1_HUMAN CANP 1 CANP CANP small subunit CANP1 CANPL 1 CANPL1 CAPN 1 CAPN1 Cell proliferation inducing protein 30 Cell proliferation-inducing gene 30 protein Micromolar Calpain Micromolar-calpain Mu Calpain muCANP muCL |
Images
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Fig1: ICC staining Calpain 1(green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of A431 cells with Calpain 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.