MSH2 Mouse Monoclonal Antibody [10G2]
cat.: EM1801-05
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: 10G2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 105 kDa
Isotype: IgG2b
Immunogen: Synthetic peptide within N-terminal human MSH2.
Positive control: HeLa cell lysate, HEK-293 cell lysate, A549 cell lysate, A431 cell lysate, HCT 116 cell lysate, SW480 cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human breast cancer tissue, human colon cancer tissue, human stomach cancer tissue, human appendix tissue, human appendicular lymph nodes tissue, mouse colon tissue, rat colon tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
1:1,000-1:5,000
1:1,000
1:200
Uniprot #: SwissProt: P43246 Human | P43247 Mouse | P54275 Rat
Alternative names: BAT26 COCA 1 COCA1 DNA mismatch repair protein Msh2 FCC 1 FCC1 hMSH2 HNPCC 1 HNPCC HNPCC1 LCFS2 MSH 2 Msh2 MSH2_HUMAN MutS homolog 2 MutS homolog 2 colon cancer nonpolyposis type 1 MutS protein homolog 2
Images
EM1801-05_1.jpg Fig1: Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HEK-293 cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: A431 cell lysate (15 µg/Lane)
Lane 5: HCT 116 cell lysate (15 µg/Lane)
Lane 6: SW480 cell lysate (15 µg/Lane)
Lane 7: PC-3M cell lysate (15 µg/Lane)
Lane 8: LNCaP cell lysate (negative) (15 µg/Lane)
Lane 9: LoVo cell lysate (negative) (15 µg/Lane)
Lane 10: NIH/3T3 cell lysate (15 µg/Lane)
Lane 11: RAW264.7 cell lysate (15 µg/Lane)
Lane 12: PC-12 cell lysate (15 µg/Lane)
Lane 13: Mouse testis tissue lysate (25 µg/Lane)
Lane 14: Rat testis tissue lysate (25 µg/Lane)

Predicted band size: 105 kDa
Observed band size: 105 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-05) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1801-05_2.jpg Fig2: All lanes: Western blot analysis of MSH2 with anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.
Lane 1: Wild-type SCC7 whole cell lysate.
Lane 2: MSH2 knockout SCC7 whole cell lysate.

EM1801-05 was shown to specifically react with MSH2 in Wild-type SCC7 cells. No band was observed when MSH2 knockout sample was tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MSH2 antibody (EM1801-05, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
EM1801-05_3.jpg Fig3: Western blot analysis of MSH2 on different lysates with Mouse anti-MSH2 antibody (EM1801-05) at 1/5,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: A431 cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: Mouse testis tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (20 µg/Lane)

Predicted band size: 105 kDa
Observed band size: 105 kDa

Exposure time: 28 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-05) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1801-05_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-05) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-05_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-05) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-05_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-05) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-05_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-05) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-05_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human appendicular lymph nodes tissue with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-05) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-05_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-05) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-05_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-MSH2 antibody (EM1801-05) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-05) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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