Anti-MSH2 antibody [10G3]
cat.: EM1801-06
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: 10G3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 104 kDa
Isotype: IgG2b
Immunogen: Synthetic peptide within N-terminal residues of Human MSH2.
Positive control: K562 cells lysates, Wild-type SCC7 whole cell lysates, human breast cancer tissue, human kidney tissue, human placenta tissue, mouse colon tissue, rat brain tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P

1:2,000-1:5,000
1:100-1:500
Uniprot #: SwissProt: P43246 Human | P43247 Mouse | P54275 Rat
Alternative names: BAT26 COCA 1 COCA1 DNA mismatch repair protein Msh2 FCC 1 FCC1 hMSH2 HNPCC 1 HNPCC HNPCC1 LCFS2 MSH 2 Msh2 MSH2_HUMAN MutS homolog 2 MutS homolog 2 colon cancer nonpolyposis type 1 MutS protein homolog 2
Images
EM1801-06_1.jpg Fig1: Western blot analysis of MSH2 on K562 cells lysates using anti-MSH2 antibody.
Lane 1: Anti-MSH2 antibody, 1:500.
Lane 2: Anti-MSH2 antibody, 1:5,000.
EM1801-06_2.jpg Fig2: Western blot analysis of MSH2 on Wild-type SCC7 whole cell lysate and MSH2 knockout SCC7 whole cell lysate. Proteins at 20 µg per lane were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-06, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. Rabbit anti-HSP90 (ET1605-56) loading control were incubated for 1 hour at room temperature at 1/10,000 dilution respectively.

The cell line was provided by Ubigene Biosciences Co., Ltd., Guangzhou, China.
EM1801-06_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
EM1801-06_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
EM1801-06_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
EM1801-06_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
EM1801-06_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MSH2 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.