HLA Class 1 ABC Mouse Monoclonal Antibody [11C3]
cat.: EM1801-09
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, ICC
Clonality: Monoclonal
Clone number: 11C3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/ml.
Purification: Immunogen affinity purified.
Molecular weight: 40 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human HLA Class 1 ABC aa 1-362 / 362.
Positive control: Hela, 293T.
Subcellular location: Cell Membrane.
Recommended Dilutions:
  WB
  ICC

1:500-1:2000
1:50-1:200
Uniprot #: SwissProt: P01889 Human | P30443 Human | P30499 Human
Alternative names: Antigen presenting molecule HLA class I histocompatibility antigen, A 1 alpha chain HLAA Leukocyte antigen class I A Major histocompatibility complex, class I, A MHC class I antigen HLA A heavy chain AS HLA B protein HLA class I histocompatibility antigen, B alpha chain HLA-B HLA-B histocompatibility type HLAB leukocyte antigen class I-B lymphocyte antigen major histocompatibility complex, class I, B MHC class I antigen GN00104 MHC class I antigen HLA-B heavy chain MHC class I antigen SHCHA MHC Class I HLA heavy chain MHC HLA-B cell surface glycoprotein MHC HLA-B transmembrane glycoprotein SPDA1 1C07_HUMAN Cw-7 alpha chain D6S204 HLA class I histocompatibility antigen HLA class I histocompatibility antigen, C alpha chain HLA class I histocompatibility antigen, Cw-1 alpha chain HLA JY3 HLA-C HLA-C histocompatibility type HLA-Cw HLA-JY3 HLAC HLC C HLC-C human leukocyte antigen-C alpha chain major histocompatibility......
Images
EM1801-09_1.jpg Fig1: Western blot analysis of HLA Class 1 ABC on Hela lysate. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal ECL Western Blotting Substrate (Product # K1801).
EM1801-09_2.jpg Fig2: Immunocytochemistry staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Ki67 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.