HLA Class 1 ABC Mouse Monoclonal Antibody [11C3]
cat.: EM1801-09
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: 11C3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human HLA Class 1 ABC aa 1-362 / 362.
Positive control: CAL-62 cell lysate, HeLa cell lysate, HepG2 cell lysate, human liver tissue, 293T.
Subcellular location: Cell Membrane, Endoplasmic reticulum membrane.
Recommended Dilutions:

Uniprot #: SwissProt: P04439 Human | P01889 Human | P10321 Human
Alternative names: Antigen presenting molecule HLA class I histocompatibility antigen, A 1 alpha chain HLAA Leukocyte antigen class I A Major histocompatibility complex, class I, A MHC class I antigen HLA A heavy chain AS HLA B protein HLA class I histocompatibility antigen, B alpha chain HLA-B HLA-B histocompatibility type HLAB leukocyte antigen class I-B lymphocyte antigen major histocompatibility complex, class I, B MHC class I antigen GN00104 MHC class I antigen HLA-B heavy chain MHC class I antigen SHCHA MHC Class I HLA heavy chain MHC HLA-B cell surface glycoprotein MHC HLA-B transmembrane glycoprotein SPDA1 1C07_HUMAN Cw-7 alpha chain D6S204 HLA class I histocompatibility antigen HLA class I histocompatibility antigen, C alpha chain HLA class I histocompatibility antigen, Cw-1 alpha chain HLA JY3 HLA-C HLA-C histocompatibility type HLA-Cw HLA-JY3 HLAC HLC C HLC-C human leukocyte antigen-C alpha chain major histocompatibility......
EM1801-09_1.jpg Fig1: Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-09) at 1/1,000 dilution.

Lane 1: CAL-62 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-09) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1801-09_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-09) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-09) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-09_3.jpg Fig3: Immunocytochemistry staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HLA Class 1 ABC polyclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).