HLA Class 1 ABC Mouse Monoclonal Antibody [11C3]
cat.: EM1801-09
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 11C3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human HLA Class 1 ABC aa 1-362 / 362. |
| Positive control: | Hela cell lysate, 293T. |
| Subcellular location: | Cell Membrane, Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2000 1:50-1:200 |
| Uniprot #: | SwissProt: P01889 Human | P30443 Human | P30499 Human |
| Alternative names: | Antigen presenting molecule HLA class I histocompatibility antigen, A 1 alpha chain HLAA Leukocyte antigen class I A Major histocompatibility complex, class I, A MHC class I antigen HLA A heavy chain AS HLA B protein HLA class I histocompatibility antigen, B alpha chain HLA-B HLA-B histocompatibility type HLAB leukocyte antigen class I-B lymphocyte antigen major histocompatibility complex, class I, B MHC class I antigen GN00104 MHC class I antigen HLA-B heavy chain MHC class I antigen SHCHA MHC Class I HLA heavy chain MHC HLA-B cell surface glycoprotein MHC HLA-B transmembrane glycoprotein SPDA1 1C07_HUMAN Cw-7 alpha chain D6S204 HLA class I histocompatibility antigen HLA class I histocompatibility antigen, C alpha chain HLA class I histocompatibility antigen, Cw-1 alpha chain HLA JY3 HLA-C HLA-C histocompatibility type HLA-Cw HLA-JY3 HLAC HLC C HLC-C human leukocyte antigen-C alpha chain major histocompatibility...... |
Images
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Fig1: Western blot analysis of HLA Class 1 ABC on Hela cell lysate. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/2,000 dilution in 5% BSA at room temperature for 2 hours, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal ECL Western Blotting Substrate (Product # K1801). |
|
Fig2: Immunocytochemistry staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HLA Class 1 ABC polyclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.