HLA Class 1 ABC Mouse Monoclonal Antibody [11C2]
cat.: EM1801-10
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 11C2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human HLA Class 1 B aa 1-362 / 362. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, 293T, human liver cancer tissue, human kidney tissue, mouse testis tissue, human liver tissue. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane . |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P04439 Human | P01889 Human | P10321 Human |
| Alternative names: | Antigen presenting molecule HLA class I histocompatibility antigen, A 1 alpha chain HLAA Leukocyte antigen class I A Major histocompatibility complex, class I, A MHC class I antigen HLA A heavy chain AS HLA B protein HLA class I histocompatibility antigen, B alpha chain HLA-B HLA-B histocompatibility type HLAB leukocyte antigen class I-B lymphocyte antigen major histocompatibility complex, class I, B MHC class I antigen GN00104 MHC class I antigen HLA-B heavy chain MHC class I antigen SHCHA MHC Class I HLA heavy chain MHC HLA-B cell surface glycoprotein MHC HLA-B transmembrane glycoprotein SPDA1 1C07_HUMAN Cw-7 alpha chain D6S204 HLA class I histocompatibility antigen HLA class I histocompatibility antigen, C alpha chain HLA class I histocompatibility antigen, Cw-1 alpha chain HLA JY3 HLA-C HLA-C histocompatibility type HLA-Cw HLA-JY3 HLAC HLC C HLC-C human leukocyte antigen-C alpha chain major histocompatibility...... |
Images
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Fig1:
Western blot analysis of HLA Class 1 ABC on different lysates with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 2 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-HLA Class 1 ABC antibody (EM1801-10) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-10) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.