APE1 Mouse Monoclonal Antibody [12H1]
cat.: EM1801-11
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: 12H1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within Human APE1 aa 20-318/318.
Positive control: HeLa cell lysate, LNCaP cell lysate, HEK-293 cell lysate, HL-60 cell lysate, A431 cell lysate, mouse brain tissue lysate, C6 cell lysate, rat testis tissue lysate, human kidney tissue, mouse liver tissue, rat colon tissue, SiHa.
Subcellular location: Nucleus. Endoplasmic reticulum, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:5,000
1:50
1:1,000
1:50-1:100
Uniprot #: SwissProt: P27695 Human | P28352 Mouse | P43138 Rat
Alternative names: AP endonuclease 1 AP endonuclease class I AP lyase APE 1 APE APE-1 APEN APEX 1 APEX APEX nuclease (multifunctional DNA repair enzyme) 1 Apex nuclease 1 APEX nuclease APEX1 APEX1_HUMAN Apurinic endonuclease Apurinic-apyrimidinic endonuclease 1 Apurinic/apyrimidinic (abasic) endonuclease Apurinic/apyrimidinic endonuclease 1 Apurinic/apyrimidinic exonuclease APX BAP1 Deoxyribonuclease (apurinic or apyrimidinic) DNA (apurinic or apyrimidinic site) lyase DNA-(apurinic or apyrimidinic site) lyase, mitochondrial EC 4.2.99.18 HAP 1 HAP1 Human Apurinic endonuclease 1 MGC139790 Multifunctional DNA repair enzyme Redox factor 1 Redox factor-1 REF 1 REF 1 protein REF-1 REF1 REF1 protein
Images
EM1801-11_1.jpg Fig1: Western blot analysis of APE1 on different lysates with Mouse anti-APE1 antibody (EM1801-11) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: LNCaP cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: HL-60 cell lysate (20 µg/Lane)
Lane 5: A431 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 45 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1801-11_2.jpg Fig2: ICC staining APE1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with APE1 monoclonal antibody at a dilution of 1/50 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
EM1801-11_3.jpg Fig3: ICC staining APE1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with APE1 monoclonal antibody at a dilution of 1/50 for 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
EM1801-11_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-APE1 antibody (EM1801-11) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-11) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-11_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-APE1 antibody (EM1801-11) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-11) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-11_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-APE1 antibody (EM1801-11) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-11) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1801-11_7.jpg Fig7: Flow cytometric analysis of APE1 was done on SiHa cells. The cells were fixed, permeabilized and stained with APE1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.