HSPA1L Mouse Monoclonal Antibody [7D3]
cat.: EM1801-16
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 7D3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein with Human HSPA1L aa 335-639 / 641. |
| Positive control: | Mouse testis tissue lysate, rat testis tissue lysate, A549 cell lysates, HeLa, human testis tissue, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Cytosol, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000 1:100 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P34931 Human | P16627 Mouse | P55063 Rat |
| Alternative names: | Heat shock 70 kDa protein 1 Hom Heat shock 70 kDa protein 1 like Heat shock 70 kDa protein 1-Hom Heat shock 70 kDa protein 1-like Heat shock 70 kDa protein 1L Heat shock 70kD protein like 1 HS71L_HUMAN HSP70 1L HSP70 HOM HSP70-Hom HSPA1L hum70t Spermatid specific heat shock protein 70 |
Images
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Fig1:
Western blot analysis of HSPA1L on different lysates with Mouse anti-HSPA1L antibody (EM1801-16) at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-16) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of HSPA1L on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1/500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: Anti-HSPA1L Antibody. Lane 2: Anti-HSPA1L Antibody, preincubated with immunization protein. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling HSPA1L with Mouse anti-HSPA1L antibody (EM1801-16) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HSPA1L antibody (EM1801-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-HSPA1L antibody (EM1801-16) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-HSPA1L antibody (EM1801-16) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-HSPA1L antibody (EM1801-16) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Flow cytometric analysis of HeLa cells labeling HSPA1L. Cells were fixed and permeabilized. Then stained with the primary antibody (EM1801-16, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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