GST3 Mouse Monoclonal Antibody [11D5]
cat.: EM1801-18
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 11D5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human GST3 aa 1-183 / 210. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HT-29 cell lysate, Jurkat cell lysate, K-562 cell lysate, JAR cell lysate, human brain tissue lysate, human lung tissue lysate, human testis tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:10,000 |
| Uniprot #: | SwissProt: P09211 Human |
| Alternative names: | Deafness Deafness X-linked 7 DFN7 FAEES3 Fatty Acid Ethyl Ester Synthase III Glutathione S Transferase 3 Glutathione S Transferase Pi Glutathione S-transferase P Glutathione S-transferase pi 1 GST class-pi GST3 GSTP Gstp1 GSTP1-1 GSTP1_HUMAN PI X linked 7 |
Images
|
Fig1:
Western blot analysis of GST3 on different lysates with Mouse anti-GST3 antibody (EM1801-18) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-18) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-GST3 antibody (EM1801-18) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1801-18) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of GST3 on different lysates with Mouse anti-GST3 antibody (EM1801-18) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si GST3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.