NQO1 Mouse Monoclonal Antibody [13A1]
cat.: EM1801-21
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 13A1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human NQO1 aa 1-274 / 274. |
| Positive control: | A549 cell lysate, SiHa cell lysate, HepG2 cell lysate, MCF7 cell lysate, HeLa cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P15559 Human | P05982 Rat |
| Alternative names: | Azoreductase Cytochrome b 5 reductase DHQU DIA 4 DIA4 Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) Diaphorase (NADH/NADPH) Diaphorase 4 Dioxin inducible 1 DT diaphorase DT-diaphorase DTD Menadione reductase NAD(P)H dehydrogenase [quinone] 1 NAD(P)H dehydrogenase quinone 1 NAD(P)H menadione oxidoreductase 1 dioxin inducible NAD(P)H quinone dehydrogenase 1 NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 NAD(P)H:menadione oxidoreductase 1 NAD(P)H:Quinone acceptor oxidoreductase type 1 NAD(P)H:quinone oxidoreductase 1 NAD(P)H:quinone oxireductase NMOR 1 NMOR I NMOR1 NMORI NQO 1 NQO1 NQO1_HUMAN Phylloquinone reductase QR 1 QR1 Quinone reductase 1 |
Images
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Fig1:
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: SiHa cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: Saos-2 cell lysate (low expression) Lysates/proteins at 30 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (EM1801-21) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si NQO1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-21) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.