Anti-Cathepsin D antibody [13F3]
cat.: EM1901-02
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: 13F3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 28/43/46 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human Cathepsin D aa 1-432.
Positive control: MCF-7, human liver tissue, human liver cancer tissue.
Subcellular location: Lysosome. Melanosome. Secreted, extracellular space.
Recommended Dilutions:
  WB
  IHC-P

1:10,000-1:50,000
1:50-1:200
Uniprot #: SwissProt: P07339 Human
Alternative names: CatD antibody CATD_HUMAN antibody Cathepsin D antibody Cathepsin D heavy chain antibody CD antibody Ceroid lipofuscinosis neuronal 10 antibody CLN10 antibody CPSD antibody ctsd antibody Epididymis secretory sperm binding protein Li 130P antibody HEL S 130P antibody Lysosomal aspartyl peptidase antibody Lysosomal aspartyl protease antibody MGC2311 antibody
Images
EM1901-02_1.jpg Fig1: Western blot analysis of Cathepsin D on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:50,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1901-02_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1901-02) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
EM1901-02_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cathepsin D antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (EM1901-02) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.