Cathepsin D Recombinant Mouse Monoclonal Antibody [13F4-R]
cat.: EM1901-03
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 13F4-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Cathepsin D aa 1-432. |
| Positive control: | MCF7 cell lysate, U-937 cell lysate, SK-Br-3 cell lysate, HepG2 cell lysate, A431 cell lysate, Human liver tissue lysate, human liver tissue, human liver cancer tissue, human breast cancer tissue. |
| Subcellular location: | Lysosome. Melanosome. Secreted, extracellular space. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P07339 Human |
| Alternative names: | CatD CATD_HUMAN Cathepsin D Cathepsin D heavy chain CD Ceroid lipofuscinosis neuronal 10 CLN10 CPSD ctsd Epididymis secretory sperm binding protein Li 130P HEL S 130P Lysosomal aspartyl peptidase Lysosomal aspartyl protease MGC2311 |
Images
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Fig1:
Western blot analysis of Cathepsin D on different lysates with Mouse anti-Cathepsin D antibody (EM1901-03) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: U-937 cell lysate (20 µg/Lane) Lane 3: SK-Br-3 cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: Human liver tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45/28 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-03) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cathepsin D antibody (EM1901-03) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-03) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Cathepsin D antibody (EM1901-03) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-03) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Cathepsin D antibody (EM1901-03) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-03) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.