Anti-Hip1 antibody [13E1]
cat.: EM1901-04
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Monoclonal
Clone number: 13E1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 116 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human Hip1 aa 8-31.
Positive control: SH-SY5Y, A549, rat brain tissue, human colon cancer tissue, human prostate cancer tissue, mouse brain tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O00291 Human | Q8VD75 Mouse
Alternative names: 2610109B09Rik antibody A930014B11Rik antibody E130315I21Rik antibody HIP 1 antibody HIP I antibody HIP-1 antibody HIP-I antibody hip1 antibody HIP1/PDGFRB fusion gene antibody HIP1/PDGFRB fusion gene, included antibody HIP1_HUMAN antibody HIPI antibody Huntingtin interacting protein 1 antibody Huntingtin Interacting Protein HIP1 antibody Huntingtin-interacting protein 1 antibody Huntingtin-interacting protein I antibody ILWEQ antibody KIAA4113 antibody MGC126506 antibody MGC27616 antibody mKIAA4113 antibody
Images
EM1901-04_1.jpg Fig1: Western blot analysis of Hip1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SH-SY5Y cell lysate
Lane 2: A549 cell lysate
EM1901-04_2.jpg Fig2: ICC staining of Hip1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-04, 1/50 dilution) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
EM1901-04_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
EM1901-04_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
EM1901-04_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
EM1901-04_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.