Hip1 Mouse Monoclonal Antibody [13E1]
cat.: EM1901-04
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: 13E1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 116 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human Hip1 aa 1-50 / 1,029.
Positive control: HCT 116 cell lysates, SH-SY5Y, rat brain tissue, human colon cancer tissue, human prostate cancer tissue, mouse brain tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O00291 Human | Q8VD75 Mouse
Entrez Gene: 192154 Rat
Alternative names: 2610109B09Rik A930014B11Rik E130315I21Rik HIP 1 HIP I HIP-1 HIP-I hip1 HIP1/PDGFRB fusion gene HIP1/PDGFRB fusion gene, included HIP1_HUMAN HIPI Huntingtin interacting protein 1 Huntingtin Interacting Protein HIP1 Huntingtin-interacting protein 1 Huntingtin-interacting protein I ILWEQ KIAA4113 MGC126506 MGC27616 mKIAA4113
Images
EM1901-04_1.jpg Fig1: Western blot analysis of Hip1 on HCT 116 cell lysates with Mouse anti-Hip1 antibody (EM1901-04) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 116 kDa
Observed band size: 116 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1901-04_2.jpg Fig2: ICC staining of Hip1 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-04, 1/50 dilution) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
EM1901-04_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
EM1901-04_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
EM1901-04_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
EM1901-04_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hip1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with primary antibody (EM1901-04, 1/200 dilution) for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.